Conjugation-Mediated Killing Technology to Treat Gram-Negative Bacteria

Gram negative bacteria cause numerous infections including pneumonia, bloodstream infections, wound or surgical site infections, and meningitis. These bacteria are resistant to multiple drugs and are increasingly resistant to most available antibiotics.

Gram-negative bacteria include many common pathogens including Pseudomonas aeruginosa, Acinetobacter baumannii, and a large family of related enteric bacteria including Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, and many others. Gram-negative bacterial infections can be as benign as Swimmer’s Ear to very serious diseases such as food poisoning, meningitis, Legionnaires’ disease, plague, cholera, dysentery, and sexually transmitted diseases such as gonorrhea.

Infections caused by Gram-negative bacteria can be difficult to treat because of their unique features, including the nature of their cell wall which makes them resistant to several classes of antibiotics.

Certain types of Gram-negative bacteria have become increasingly resistant to nearly all available antibiotic drugs. This has also increased illness and death from bacterial infections, heightened the challenge for healthcare facilities, and contributed to escalating healthcare costs.



Atterx Biotherapeutics Gram Negative vs Gram Positive

How it Works

Atterx Biotherapeutics GN-4474: How it Works



GN-4474 Reduces Established Infection 100X in 24 hours

Atterx Biotherapeutics GN-4474 Efficacy Chart 1

Treatment with GN-4474 () reduced infection by an average of 98%, and half the animals showed no signs of infection compared to untreated controls ().

Furthermore, treatment with GN-4474 prevented sepsis in the same animals (not shown).

Gram-negative Bacteria Do Not Develop Resistance

Atterx Biotherapeutics GN-4474 Efficacy Chart 2

  • Pathogenic bacteria were exposed to GN-4474 in vitro; surviving bacteria were re-exposed to GN-4474, repeatedly up to 20 times, to try and force the development of resistance.
  • If the bacteria became resistant, survival would increase and the blue line () would trend upwards towards the turquoise line () controls over time.
  • There is no evidence that these pathogenic bacteria are developing resistance to GN-4474.